He is Product Manager for Malvern Panalytical light scattering and micro-calorimetry platforms and Pall Fortébio product lines. He works for Alfatest since 2015, focusing on the molecular interactions and nanoparticle/protein characterization instrumentation. Andrea Pigozzo is graduated in Environmental Sciences at University Ca’ Foscari of Venice. New Zetasizer ULTRA live demonstration (replicate)Īndrea Pigozzo - Alfatest srl – Distributore Malvern Panalytical per l’Italiaĭr. See the whole nanoparticle sample with MADLS ®: particle characterization you can trust and easy, calibration-free number concentration measurements (replicate) Reliable DLS size data quicker with Adaptive Correlation (replicate) See the whole nanoparticle sample with MADLS ®: particle characterization you can trust and easy, calibration-free number concentration measurements Reliable DLS size data quicker with Adaptive Correlation PROGRAMME: 2 replicate sessions at 9.15 and 11.00 The new Zetasizer ULTRA will be presented on site during a live demonstration. The workshop will be dedicated both to measurement principle and applicative examples to illustrate the benefits of Adaptive correlation and MADLS ® respect to classical DLS. The brand new Zetasizer ULTRA from Malvern Panalytical featuring these 2 techniques will be officially presented for the first time in Italy during this worshop. The workshop will be dedicated to the brand new and revolutionary Adaptive correlation and multi-angle Dynamic Light scattering MADLS ® techniques for more complete particle size distributions and calibration-free concentration analysis. 7 Zeta potential quality report for the Zetasizer Nano high, then the laser beam will become attenuated by the particles reducing the scattered light that is being detected. Light scattering is a primary technique for this purpose, and Malvern Panalytical is the leader provider of light scattering instruments for nanoparticle characterization. Overall, a useful method for tunable, stabilizer-free PLGA nanoparticle formulation was developed for use in drug and vaccine delivery, and immune targeting.The ultimate technology for nanoparticle size and number concentration analysisįaculty of Civil and Industrial EngineeringĬharacterizing size and concentration is a key step in the study and development of dispersed nanosystems and for the control of the final product properties and quality. Variation in particle size was achieved through altering PLGA stoichiometry while maintaining the ability to encapsulate DNA and were modified with elastin-like polymers for increased immune tolerance. Subsequent experiments determined P4 nanoparticles were as stable as those made with PVA, yet significantly less cytotoxic. The nanoparticles showed larger diameter when loaded with HDN as. Mean size distribution of GA-AgNPs and GA-AgNPs-HDN were found to be 107 and 182 nm respectively. We find that under physiological conditions (PBS at 37˚C), the most stable PLGA formulation (P4) was found to contain a greater L:G ratio (65:35), lower MW, and carboxyl terminus. Figure Legend Snippet: ( A, B ) Size distribution histogram of GA-AgNPs and GA-AgNPs-HDN respectively which were obtained from zetasizer analysis on Malvern zetasizer instrument. In 2003, the Zetasizer Nano family, incorporating patented non-invasive backscatter (NIBS) technology, was launched.
A suite of PLGAs were fabricated using solvent evaporation methods and assessed for particle size and stability in water. Scattering (ELS) by Malvern Instruments in the early 1980s resulted in the world’s first integrated particle size and zeta potential measurement system, the Zetasizer 2.
Here, we developed a tunable, stabilizer-free PLGA nanoparticle formulation capable of encapsulating plasmid DNA and demonstrated the formation of an elastin-like polymer PLGA hybrid nanoparticle with exceptional stability and biocompatibility. While the use of toxic stabilizers such as polyvinyl alcohol (PVA) limit the utility of PLGA, stabilizer-free PLGA nanoparticles are rarely used because they can be challenging to prepare. Biocompatible nanoparticles composed of poly(lactic-co-glycolic acid) (PLGA) are used as drug and vaccine delivery systems because of their tunability in size and sustained release of cargo molecules.